Activity and Diversity of Methanotrophic Bacteria at Methane Seeps in Eastern Lake Constance Sediments

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Activity and diversity of methanotrophic bacteria at methane seeps in eastern Lake Constance sediments.

The activity and community structure of aerobic methanotrophic communities were investigated at methane seeps (pockmarks) in the littoral and profundal zones of an oligotrophic freshwater lake (Lake Constance, Germany). Measurements of potential methane oxidation rates showed that sediments inside littoral pockmarks are hot spots of methane oxidation. Potential methane oxidation rates at littor...

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Abundance and activity of methanotrophic bacteria in littoral and profundal sediments of lake constance (Germany).

The abundances and activities of aerobic methane-oxidizing bacteria (MOB) were compared in depth profiles of littoral and profundal sediments of Lake Constance, Germany. Abundances were determined by quantitative PCR (qPCR) targeting the pmoA gene and by fluorescence in situ hybridization (FISH), and data were compared to methane oxidation rates calculated from high-resolution concentration pro...

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Aerobic methanotrophic bacterial communities in sediments of Lake Constance

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Aerobic and Anaerobic Oxidation of Methane in Sediments of Lake Constance

13 Introduction 13 Materials and Methods 15 Results 19 Discussion 23 Acknowledgements 29 Chapter 3 Activity and Diversity of Methanotrophic Bacteria at Methane Seeps in Eastern Lake Constance Sediments 30 Abstract 30 Introduction 30 Materials and methods 31 Results 35 Discussion 42 Acknowledgements 4530 Introduction 30 Materials and methods 31 Results 35 Discussion 42 Acknowledgements 45

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Preferential cultivation of type II methanotrophic bacteria from littoral sediments (Lake Constance).

Most widely used medium for cultivation of methanotrophic bacteria from various environments is that proposed in 1970 by Whittenbury. In order to adapt and optimize medium for culturing of methanotrophs from freshwater sediment, media with varying concentrations of substrates, phosphate, nitrate, and other mineral salts were used to enumerate methanotrophs by the most probable number method. Hi...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 2011

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.02776-10